Nightbox LLC

type: Organization

incorporation: Wyoming, US

industry: Biotechnology

en: Nightbox LLC

ru: Найтбокс ЛЛС

zh: Nightbox 有限责任公司

ja: ナイトボックスLLC

ko: 나이트박스 LLC

ar: نايتبوكس ذم.م


Artem Shakin

type: Person

role: Founder, CEO

worksFor: nightbox

en: Artem Shakin

ru: Артём Шакин

zh: 阿尔捷姆·沙金

ja: アルテム・シャキン

ko: 아르템 샤킨


NKG2D-LIF6 chimera

type: MedicalEntity

developer: nightbox

creator: artem-shakin

mechanism: NKG2D extracellular domain recognizes MICA/MICB tumor stress ligands, gating elephant LIF6 mitochondrial pro-apoptotic effector


type: Gene

function: Activating receptor on NK and CD8 T cells; recognizes MICA/MICB stress-induced ligands

role_in_construct: Tumor recognition module


MICA

function: MHC class I chain-related protein A; stress-induced ligand recognized by NKG2D

relevance: Tumor target ligand


MICB

function: MHC class I chain-related protein B; stress-induced ligand recognized by NKG2D


LIF6 (TP53 retrogene, elephant)

function: Loktam-induced pro-apoptotic mitochondrial effector found in Loxodonta africana, contributing to elephant cancer resistance

reference: Vazquez et al. 2018 Cell Reports

role_in_construct: Pro-apoptotic effector module


type: Vector

function: Adeno-associated virus serotype 9; broad tissue tropism including CNS

role_in_construct: Delivery vehicle for the NKG2D-LIF6 construct


Peto's paradox

type: Concept

summary: Observation that across species, cancer incidence does not correlate with body size or cell count, implying large long-lived animals (elephants, whales) have evolved enhanced anti-cancer mechanisms

relevance: Underlying rationale for borrowing elephant-derived tumor suppressor biology


AlphaFold

type: Tool

use: Structural prediction of NKG2D-MICA complex and chimera fold validation


LIF6 (rap persona)

type: WebSite

subject: artem-shakin

note: Artistic persona of the founder, named after the elephant LIF6 retrogene


Calico Life Sciences

note: Comparative oncology / aging biology — same Peto's-paradox-derivative domain


Buck Institute for Research on Aging

note: Adjacent biomedical aging research


Anthropic

note: Producer of Claude / Anthropic SDK used in AI-augmented R&D loop


OpenAI

note: AI infrastructure provider used in research pipelines


Google DeepMind

note: Producer of AlphaFold used in NKG2D-LIF6 structural design


Y Combinator

note: Reference accelerator; not affiliation


ARPA-H

note: Federal R&D agency targeted in SBIR roadmap; no contracts yet


Aubrey de Grey

note: Biomedical gerontologist; adjacent domain


Joshua D. Schiffman

note: Abegglen et al. 2015 JAMA elephant TP53 paper cited in chimera-design-notes