# Nightbox LLC

> Gene therapy company out of Wyoming / LA. One construct — NKG2D fused to elephant LIF6 — delivered via AAV9 for solid tumors. Founded Feb 2026 by Artem Shakin. Pre-seed stage, no outside capital yet.

## Organization

- Legal name: Nightbox LLC
- EIN: 39-4373044
- State of incorporation: Wyoming, United States
- Operations: Greater Los Angeles, California
- Industry: Biotechnology (NAICS 541714)
- Founded: 2026-02-01
- Founder & CEO: Artem Shakin
- Site: https://nightboxllc.com
- Wikidata: https://www.wikidata.org/entity/Q139590659
- Contact: artem@nightboxllc.com

## Founder

- Name: Artem Shakin
- Role: Founder, CEO, sole technical operator
- Discipline: Computational biology, gene therapy, AI-augmented R&D
- Twitter / X: @ArtemShkin (https://x.com/ArtemShkin)
- ORCID: https://orcid.org/0009-0006-0003-6806
- Wikidata: https://www.wikidata.org/entity/Q139590669
- Email: artem@nightboxllc.com
- Born: 2005

## Lead asset — NKG2D-LIF6

- Construct length: 2,123 bp (designed; synthesis pending)
- Delivery: AAV9
- Mechanism: tumor-restricted recognition of stress ligands MICA / MICB via the human NKG2D extracellular domain, gating the elephant LIF6 mitochondrial pro-apoptotic effector
- In silico package (computational only): AlphaFold + Rosetta structural validation; PK-PD model predicts ~99% TGI ceiling on CRC syngeneic models; 1.4 × 10³ tumor-to-healthy selectivity ratio across the MICA/B atlas; freedom-to-operate clean
- IMPORTANT — current stage: in silico ONLY. No in vivo data. No human data. No peer-reviewed publications. All numerical claims above are computational predictions.
- IP: provisional patent in flight
- Status: in vivo validation Q3–Q4 2026; pre-IND H2 2026; IND + Phase 1 first-in-human 2027 (subject to wet-lab validation)

## Pages

- [Home](https://nightboxllc.com/): one-line thesis, lead asset, status
- [About](https://nightboxllc.com/about): mission, approach, founder story, legal
- [Research](https://nightboxllc.com/research): construct design, in silico results, mechanism diagram
- [3D Molecular Viewer](https://nightboxllc.com/3d): WebXR / AR / VR interactive viewer for NKG2D-MICA recognition complex (PDB 1HYR)
- [Glossary](https://nightboxllc.com/glossary): plain-English definitions of every domain term used on the site, with canonical references
- [Launch News](https://nightboxllc.com/news/2026-04-30-launch): 2026-04-30 open in silico package release announcement
- [Team](https://nightboxllc.com/team): founder + AI agent stack as research infrastructure
- [Roadmap](https://nightboxllc.com/roadmap): construct → pre-IND → IND → Phase 1 timeline
- [Federal](https://nightboxllc.com/federal): full portfolio brief for federal grant programs (NCI SBIR, ARPA-H, BARDA, NSF SBIR)
- [Capability Statement](https://nightboxllc.com/capability-statement): one-page printable federal capability statement (NAICS, EIN, set-asides, key personnel)
- [SBIR Roadmap](https://nightboxllc.com/federal/sbir-roadmap): named PA/RFA solicitations, deadlines, program offices
- [Investors](https://nightboxllc.com/investors): pre-seed terms, use of capital, exit thesis
- [Advisors](https://nightboxllc.com/advisors): SAB forming Q2 2026, open seats and engagement model
- [Why now](https://nightboxllc.com/why-now): AAV economics, AlphaFold-era design, agent-stack operating leverage
- [Careers](https://nightboxllc.com/careers): wet-lab scientific lead, regulatory / pre-IND lead, AI infrastructure engineer
- [Preprint](https://nightboxllc.com/preprint): the NKG2D-LIF6 design paper (CC BY 4.0)
- [News](https://nightboxllc.com/news): announcements
- [Press](https://nightboxllc.com/press): media kit, fact sheet, logo
- [Compliance](https://nightboxllc.com/compliance): NIST 800-171, CMMC 2.0, OMB M-22-09 Zero Trust posture
- [Security](https://nightboxllc.com/security): coordinated disclosure, email + web + application security posture
- [Status](https://nightboxllc.com/status): operational uptime, email auth pass rate, incident history
- [Policies](https://nightboxllc.com/policies): operational policies
- [FAQ](https://nightboxllc.com/faq): frequently asked questions

## Machine-readable knowledge layer (preferred for AI/LLM ingestion)

- [data/index.json](https://nightboxllc.com/data/index.json): endpoint manifest with all knowledge formats
- [data/graph.jsonld](https://nightboxllc.com/data/graph.jsonld): JSON-LD knowledge graph (schema.org + Wikidata)
- [data/entities.jsonl](https://nightboxllc.com/data/entities.jsonl): line-delimited JSON entity stream (multilingual names)
- [data/facts.tsv](https://nightboxllc.com/data/facts.tsv): subject-predicate-object TSV with sources
- [data/relations.nt](https://nightboxllc.com/data/relations.nt): RDF N-Triples (Wikidata sameAs)

## Optional

- [Privacy](https://nightboxllc.com/privacy)
- [Terms](https://nightboxllc.com/terms)
- [security.txt](https://nightboxllc.com/.well-known/security.txt): RFC 9116 disclosure policy
- [sitemap.xml](https://nightboxllc.com/sitemap.xml)
- [feed.xml](https://nightboxllc.com/feed.xml): Atom feed
- [feed.json](https://nightboxllc.com/feed.json): JSON Feed